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ATCCCRL-2276 Dubca
基本信息
资源编号 ATCCCRL-2276
资源名称 Dubca
种属 Camelus dromedarius, camel skin
类型 SV40 transformed
形态 fibroblast
提供形式 P1-2代冻存管
致病类型 Autopsy
安全等级 2
模式菌株 未知
应用领域 SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
The cells may be used for virus diagnosis and vaccine production.
培养方法
培养基 The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法 Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the flask to set at room temperature (or incubate at 37C) until cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
生长特性 monlayer
存储条件 Culture medium, 95%; DMSO, 5%
详细说明
Age 2 months gestation
Gender female
Derivation The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Clinical Data female
Virus Resistance parapox virus; canine adenovirus type 1 (CAV); Aleutian disease virus (ADV); influenza virus (A equi 1); ECBO virus, equine arteritis virus (EAV)
Passage History Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
描述 The cell line designated Dubca (Dubai Camel) was originated in 1990 from a specimen taken from a fetus during the autopsy of the mother at the Veterinary Research Laboratory in Dubai, UAE.
Cells at passage 7 were transformed by infection with SV40 and the resulting cells were cloned in soft agar.
SV40 large T-antigen is detected by Western blotting.
The cell line is susceptible to infection with viruses of several different families.
Orf, Newcastle disease virus (NDV) and Sindbis virus show an abortive infection with virus replicating only in the first passages.
While some isolates of bovine viral diarrhea virus (BVDV) and border disease virus (BDV) could not be grown in Dubca cells other isolates could be propagated to a limited extent (only a few cells expressed viral antigen).
The cells may be used for virus diagnosis and vaccine production.
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