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ATCC CRL-3226 VMM5A
基本信息
资源编号 ATCC CRL-3226
资源名称 VMM5A
种属 人黑色素瘤细胞
类型 Melanocyte
形态 Epithelial-like
提供形式 冻存管
致病类型 Melanoma, Stage IIIC; malignant
安全等级 1
模式菌株 未知
应用领域 Drug screening Development of targeted therapy Development of combination therapy Tumor vaccine development
说明书
产品图片
培养方法
培养基 90%RPMI-1640+10%FBS
传代方法 Volumes are for a T-75 flask; Adjust accordingly Remove and discard the cell culture medium from the flask. Rinse the cell monolayer with Dulbecco’s PBS without calcium or magnesium and remove. Add 3 to 4 ml of the trypsin-EDTA solution, rotate flask to rinse cell monolayer, remove trypsin solution, and incubate at 37oC. Once the cells appear to be detached, add 10 ml of complete growth medium with a pipette to the cell suspension to inactivate the trypsin. Gently wash any remaining cells from the growth surface of the   flask. Check the  cells with the microscope to be sure that most (>95%) are single cells. If cell clusters are apparent, continue to disperse the cells with gentle pipetting. Subculture as necessary. Place the flask back into the incubator. Examine the culture the following day to ensure the cells have reattached and are actively growing. Repeat when cells reach confluence.
生长条件 95%空气+5%二氧化碳37摄氏度
生长特性 贴壁生长
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
详细说明
Age 81
Gender Male
Ethnicity Caucasian
Derivation Derived from tumors taken from tumor-involved lymph nodes from patients at the University of Virginia
Clinical Data Primary Site Right Posterior Shoulder; Metastatic Site Lymph Node, Right Neck
Antigen Expression Positives High VEGF-R2, Low GP100, Tyrosinase, Low MAGE-A1, MMP-1
HLA Typing A2.1,A1101,B39,B44, BW4,BW6,C7(17)DR7, DR8,DR11,DQ2,DQ7
STR Profile Amelogenin X,Y D5S818 9 D7S820 9,10 D13S317 12 D16S539 12 vWA 16 TH01 9.3 TPOX 8,11 CSF1PO 11
Sterility Tests Pass
Passage History Unknown. 2 passages from 2003 frozen cell line stock, but it is not known how long the line was in culture after being established from tumor tissue obtained in 1992.
Year of Origin 1992
描述 NRAS: wt CDKN2A: wt BRAF Mutation: V600E APC Mutation PDGFRA Mutation: D842V PTEN Mutation: R173H  more sensitive to BAY43-9006 (BRAF kinase inhibitor) and to rapamycin (mTOR kinase inhibitor), compared to cell lines with wild-type B-Raf
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