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BNCC339806 人直肠腺癌细胞
基本信息
资源编号 BNCC339806
资源名称 人直肠腺癌细胞
种属 SW1463 [SW 1463, SW-1463]
类型 贴壁生长
形态 上皮样细胞
分离基物 直肠腺癌;女性
提供形式 T25细胞培养瓶/冻存管
致病类型 Dukes' type C, colorectal adenocarcinoma
安全等级 1
模式菌株 未知
说明书
产品图片
培养方法
培养基 90%L-15+10%FBS
传代方法 Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture just prior to confluency Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mof complete growth medium and aspirate cells by gently pipetting.  To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube and spin at approximately 125 x g for 5 to10 minutes. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 37°C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
生长条件 Atmosphere: Air, 100%
存储条件 90%FBS+10%DMSO
详细说明
Age 66 years
Gender female
Ethnicity Caucasian
Derivation The line was derived from a grade II mucous producing and grade III solid tumor.
Clinical Data 66 years
Caucasian
female
Antigen Expression blood type A; Rh +
Genes Expressed carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Cellular Products carcinoembryonic antigen (CEA) 223 ng/106 cells/10 days; keratin
Tumorigenic Yes
Effects Yes, in nude mice(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
STR Profile Amelogenin X
CSF1PO 11,12
D13S317 12,13
D16S539 11
D5S818 13,14
D7S820 9
THO1 6,7
TPOX 8,11
vWA 16
Isoenzymes ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1-2
描述 CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.  
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