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BNCC339761 貂肺上皮细胞
基本信息
资源编号 BNCC339761
资源名称 貂肺上皮细胞
种属 MV 1 Lu (NBL-7)
类型 贴壁生长
形态 CM8-1培养液中,贴壁,上皮细胞样,多角形、梭形
提供形式 复苏形式:T25培养瓶1瓶;或者冻存形式:2ml冻存管2支,干冰费另计。
安全等级 1
模式菌株 未知
应用领域 This cell line is a suitable transfection host and is useful for focus forming assays for murine and feline sarcoma viruses.
说明书
产品图片
培养方法
传代方法 :将旧培养液吸除,PBS清洗两遍后,加入6mL(/100mm皿)胰酶,在显微镜下观察,期间禁止摇晃培养皿,细胞刚有脱落时,则吸除大部分胰酶,留约0.5mL,移至培养箱消化,约4min取出。传代用12mL CM8-1培养液终止消化,轻轻吹打均匀细胞,后可分2~4皿培养;
生长条件 37℃,5%CO2,CM8-1培养液。CM8-1培养液:90%EMEM+10%FBS。EMEM:EMEM培养液。
生长特性 在培养过程中,DMEM-H培养细胞不增殖,要用EMEM培养基。
存储条件 冻存则用6mL冻存液(90%FBS+10%DMSO)终止消化,吹打均匀,分为6支冻存管,用程序降温盒于-80℃冻存,过夜转移至液氮中保存。
详细说明
Age near term fetus
Gender male and female mixed
Karyotype Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximately 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.
Derivation The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.
Clinical Data male and female mixed
Virus Susceptibility Herpes simplex virus Reovirus 3 Vaccinia virus Vesicular stomatitis New Jersey virus
Virus Resistance adenovirus 5; coxsackievirus A9, B5; poliovirus 2
Year of Origin May, 1964
Cross References Nucleotide (GenBank) M64428 Mustela vison TI1 mRNA, complete cds.
Nucleotide (GenBank) J02248 mink cell focus-forming 247 provirus clone 1 ltr.
Nucleotide (GenBank) J02250 mink cell focus-forming 247 provirus clone 2 ltr(seg 1).
Nucleotide (GenBank) J02251 mink cell focus-forming 247 provirus clone 2 ltr(seg 2).
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