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ATCC CCL-70 CV-1
基本信息
资源编号 ATCC CCL-70
资源名称 CV-1
种属 非洲绿猴肾细胞
形态 纤维细胞
提供形式 冻存管(ATCC)
致病类型 Normal
安全等级 1
模式菌株 未知
应用领域 efficacy testing
transfection host
viruscide testing
说明书
产品图片
培养方法
培养基 90%EMEM+10%FBS
传代方法 Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.  Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.   Resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels. Place culture vessels in incubators at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week
生长条件 95%空气+5%二氧化碳37摄氏度
生长特性 贴壁生长
存储条件 90%FBS+10%DMSO 液氮
详细说明
Age 141 days
Gender male
Karyotype This is a pseudodiploid, male African green monkey cell line. The modal chromosome number was 60, occurring in 48% of cells, and the rate of polyploidy was at 4.4%. Only a few markers were found., Of these M1, a probable deleted N11, was found in all cells examined; M3 of unknown origin was in some cells; and the remaining 2 to 3 others of unknown origins were found only once., N11 was uniformly single copied, and N16 was also single copied in most cells. Both X and Y chromosomes were also detected in every cell.
Derivation The CV-1 cell line was derived from the kidney of a male adult African green monkey by F.C. Jensen, et al. in March, 1964 for use in Rous sarcoma virus transformation studies.
Clinical Data adult African green monkey
male
Virus Susceptibility Human poliovirus 1 Herpes simplex virus Eastern equine encephalitis virus , Eastern equine encephalitis virus Western equine encephalitis virus , Western equine encephalitis virus California encephalitis virus , Melao virus SV40 virus
Year of Origin 1964
Cross References Nucleotide (GenBank) U83324 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 1, complete cds.
Nucleotide (GenBank) U83325 Cercopithecus aethiops CC chemokine receptor-5 (CCR5) gene, clone 2, complete cds.
描述 This cell line is a suitable host for transfection, especially by SV40 vectors.
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